- A comprehensive analysis of both differentially expressed gene and miRNA profiles to deepen our understanding of host-pathogen interactions.
- Microbial biotechnology with a focus on developing sustainable solutions for environmental and human health improvement
- Quality assurance of pharmaceutical products; Bioequivalence studies
Professional Expertise
Proficient in differential gene expression analysis and microRNA data interpretation related to infectious diseases. Skilled in gene enrichment analysis, utilizing diverse techniques for identifying disease-associated biological processes and pathways. Experienced in developing sequence pipelines for mRNAseq and microRNA seq, obtaining feature counts for downstream analysis. Strong background in various visualization methods, including gene ontology and pathway mapping, enhancing data comprehension and presentation.
Proficient in metagenomics, comparative genomics, transcriptomics, proteomics, and systems biology. Applied expertise in strain improvement programs, exploring novel metabolites and pathways. Enhanced research efficiency and innovation by leveraging advanced computational tools.
Analytical Skills
Proficient in metabolite analysis using GC platforms (Agilent 6890, 7890, and Perkin Elmer). Skilled in method optimization and precise compound identification through chromatogram interpretation. Experienced in HPLC analysis with Agilent 1100 series, specializing in method development, sample preparation, and accurate compound quantification. Expertise in diverse chromatographic and mass spectrometric instruments, enabling accurate metabolite analysis and valuable contributions to scientific research and analytical projects.
Books
- Perera, A. L. T. & Jayasinghe, H.S. PCR-based plant DNA fingerprinting: A Laboratory Manual, University of Peradeniya, Sri Lanka 1998.
PhD Thesis
- Rhizobial-Fungal-Phenolic Interactions in N2 Fixing Symbiosis
Referred Journal articles
- Jayasinghearachchi HS, Muthugama TA, Masakorala J, Kulasekara US, Jayaratne K, Jayatunga DADN, De Silva AD, Corea EM. Burkholderia pseudomallei in soil and natural water bodies in rural Sri Lanka: A hidden threat to public health. Front Vet Sci. 2023 Jan 17;9:1045088. doi: 10.3389/fvets.2022.1045088. (Impact factor-3.471).
- Jayasinghearachchi HS, Corea EM, Jayaratne KI, Fonseka RA, Muthugama TA, Masakorala J, Ramasinghe RY, De Silva AD. Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka. PLoS Negl Trop Dis. 2021 Dec 1;15(12):e0009917. doi: 10.1371/ journal. pntd.0009917. PMID: 34851950. [Impact factor: 411]
- Jayasinghearachchi, H. S., Francis, V. R., Sathkumara, H. D., Krishnananthasivam, S., Masakorala, J., Muthugama, T., De Silva, A. D., & Corea, E. M. (2021). Nonclonal Burkholderia pseudomallei Population in Melioidosis Case Cluster, Sri Lanka. Emerging infectious diseases, 27(11), 2955–2957. https://doi.org/10.3201/eid2711.210219
- Ariyaratne M, Gunasekara P, Wajirasena PH, Rathnayake DM, Dilani D, Chathuranga T, Gomes L, Jayatunga D, Wewita S, Meegahage T, Jayasinghearachchi HS, Wijewickrama A, Malavige G, De Silva AD. Comparison of two rapid test kits with real time polymerase chain reaction for early diagnosis of dengue in Sri Lanka. J Immunoassay Immunochem. 2021 Nov 2:1-9. doi: 10.1080/15321819.2021.1984252. Epub ahead of print. PMID: 34724872.
- Jayasinghearachchi H.S, et. al., Whole-Genome Sequences of Eight Clinical Isolates of Burkholderia pseudomallei from Melioidosis Patients in Eastern Sri Lanka. Microbiol Resour Announc. 2019 Aug 15;8(33):e00645-19. doi: 10.1128/MRA.00645-19. PMID: 31416870; PMCID: PMC6696645.
- Jayasinghearachchi, H. S. et al. Biological hydrogen production by extremely thermophilic novel bacterium Thermoanaerobacter mathranii A3N isolated from oil producing well. Int J Hydrogen Energy, 2012;37:5569-5578. doi.org/10.1016/j.ijhydene.2011.12.145.
- Jayasinghearachchi, H. S. & Lal. B. Oceanotoga teriensis gen. nov.sp. nov., a novel -thermophilc, bacterium belonging to the order Thermotogales, isolated from off-shore oil- producing well Bombayhigh in India. Int Syst Evol Microbiol, 2011;61:554-560. doi: 10.1099/ijs.0.018036-0.
- Jayasinghearachchi, H.S. et.al., Fermentative hydrogen production by new marine Clostridium amygdalinum strain C9 isolated from offshore crude oil pipeline. Int J Hydrogen Energy 2010;35:6665-6673. doi.org/10.1016/j.ijhydene.2010.04.034.
- Jayasinghearachchi, H.S. et.al., Fermentative hydrogen production by two novel strains of Enterobacter aerogenes HGN-2 and HT 34 isolated from sea buried crude oil pipelines. Int J Hydrogen Energy, 2009;34:7197-7207. doi.org/10.1016/j.ijhydene.2009.06.079.
- Jayasinghearachchi, H.S. et.al. Prevalence of duodenal ulcer promoting gene (dupA) of Helicobacter pylori in patients with duodenal ulcer disease in North Indian population. Helicobacter, 2007;12:591-597. doi: 10.1111/j.1523-5378.2007.00557.x.
- Jayasinghearachchi, H. S. & Seneviratne, G. Fungal solubilization of rock phosphate is enhanced by forming fungal-rhizobial biofilms. Soil Biology Biochem 2006;38:405-408. doi.org/10.1016/j.soilbio.2005.06.004
- Seneviratne, G. & Jayasinghearachchi, H. S. A Rhizobial biofilms with nitrogenase activity alters nutrient availability in soil. Soil Biol Biochem, 2005;37:1975-1978. doi.org/10.1016/j.soilbio.2005.02.027.
- Jayasinghearachchi, H. S. & Seneviratne, G. A bradyrhizobial-Penicillium spp. biofilm with nitrogenase activity improves N2 fixing symbiosis of soybean. Biol Fert Soils,2004;40:432-434. doi.org/10.1007/s00374-004-0796-5.
- Jayasinghearachchi, H. S. & Seneviratne, G. Can mushroom fix atmospheric nitrogen? J Biosciences, 2004;29:293-296. doi.org/10.1007/BF02702611.
- Seneviratne, G. & Jayasinghearachchi, H. S. Phenolic acids: A possible agent of modifying N2-fixing symbiosis through rhizobial alteration. Plant Soil,2003;252:385-395. doi:org/10.1023/A:1024725511783
- Seneviratne, G. & Jayasinghearachchi, H. S. Mycelial colonization by bradyrhizobia and azorhizobia. J Biosciences, 2003;2:243-247. doi: 10.1007/BF02706224
- Jayasinghearachchi, H.S. et.al., Tannin interactions with legume rhizobial N2 fixing symbiosis. Int J Agric Res, 2006;1:1-7. doi:10.3923/ijar.2006.1.7.
- Jayasinghearachchi, H.S. et.al., polyacrylamide gel electrophoretic approach of fingerprinting soil polyphenols. Int J Soil Science, 2006;1:53-57. doi: 10.3923/ijss.2006.53.57.
- Jayasinghearachchi, H. S. & Seneviratne, G.A mushroom helps improve endophytic colonization of tomato by Pseudomonas fluorescence through biofilm formation. Research Journal of Microbiology, 2006;1: 83-89. doi: 10.3923/jm.2006.83.89
- Gunasekera D.T.W, Gunawardana D, Jayasinghearachchi, H.S. Microbial diversity in nitrogen fixing nodules of Casuarina equisetifolia and its impact on plant growth and soil quality. International Journal on Multidisciplinary Studies, August 2015, USJ, Sri Lanka. doi: 10.4038/ijms.v3i1.79
Conference papers
- HS Jayasinghearachchi, DS Govindapala, WMID Nakkawita, PH Premaratne, CL Goonasekara, DGP Kawyangana, KWT Chathuranga, US Kulasekara and AD De Silva. Molecular Characterization and Phylogenetic Relationship of SARS-CoV-2 Strains Infecting a NAVY Cohort during the First Wave of the COVID-19 in Sri Lanka. Abstract publication. Proceedings of the International Research Conference KDU -2021
- Jayasinghearachi HS, Govindapala DS, Nakkawita WMID, Premaratne PH, Goonasekara CL, Jayasinghe J, Karunaratne M, KawyanganaDGP, Chathuranga KWT, Kulasekara US, De Silva AD. Prospective study on the clinical characteristics, viral positivity and antibody in an asymptomatic/ minimally symptomatic SARS- CoV2 positive Navy Cohort in Sri Lanka. Abstract publication. Proceedings of the International Research Conference KDU -2020
- Kulasekara, US; Nayanathara, O; Ranasinghe, A; Gamage, P; Ramasinghe, RY; Munasinghe, J; De Silva, AD; Premaratne, PH; Jayasinghearachchi, HS; Fernandopulle, R. Evaluating Bioequivalence of Two Formulations of Clarithromycin Immediate Release Film Coated Tablets in Healthy Sri Lankan Subjects under Fasting Conditions. Proceedings of the International Research Conference KDU -2021.
- Jayaratne, K; Jayasinghearachchi, HS; Corea, E; Fonseka, A; Muthugama, T; Masakorala, J; De Silva, AD. Unravelling the Genetic Diversity of Burkholderia pseudomallei in Sri Lanka using Multilocus Sequence Typing. Proceedings of the International Research Conference 2021.
- Jayaratne, K; Jayasinghearachchi, HS; Corea, E; Masakorala, J; De Silva, AD. Detection of Variably Present Genotypes, bimABP/bimABM, fhaB3 YLF/BTFC and LPSA in Sri Lankan Clinical Isolates of Burkholderia pseudomallei Using Real-Time PCR Based Molecular Assay. Proceedings of the International Research Conference KDU -2021
- Mathushika, J; Jayasinghearachchi, HS; Alahakoon, SB. Biodegradation of Burnt Engine Oil by Surfactant Producing Marine Isolates of Pseudomonas spp. KDU-IRC 2022.
- Piyathilaka, RD; Sooriyarachchi, TD; Kulasekara, US; Gamage, P; Munasinghe, J; Jayasekara, P; Govindapala, D; Jayasinghearachchi, HS; Fernandopulle, R. Evaluating Bioequivalence of Two Formulations of Metformin Hydrochloride US 500 mg XR Oral Tablets Under Fasting Conditions in Healthy Sri Lankan Subjects, KDU-IRC 2022. http://ir.kdu.ac.lk/handle/345/5847
- Mathushika, J; Alwis, PDNV; Nakkawita, WMID; Jayasinghearachchi, HS. The Antimicrobial Activity of Crude Extract of Pyocyanin from a Marine Isolate of Pseudomonas sp. against Clinical Strain of Staphylococcus aureus. KDU-IRC 2022. http://ir.kdu.ac.lk/handle/345/6201
- Nakkawita, WMID; Jayasinghearachchi, HS; Jayasekara, P; Pulleperuma, S; Corea, E. Melioidosis: A Deadly Disease Mimicking Leptospirosis Reported from a Highly Endemic Area of Sri Lanka. KDU-IRC. http://ir.kdu.ac.lk/handle/345/5851
- Thilini A Muthugama, Himali S Jayasinghearachchi, Jayanthi Masakorala, Sajeewani Pathirana, Ravija Y C Ramasinghe, Dharshan de Silva, Enoka M Corea Genetic diversity among Burkholderia pseudomallei isolates from neurological melioidosis in Sri Lanka (REF NO.: WMC19.094), WMC 2019, 9th World melioidosis Congress, 15-18 October, Hanoi, Vietnam.
- Thilini A Muthugama, Himali S Jayasinghearachchi, Jayanthi Masakorala, Sathkumara HD, Krishnananthasivam S, Dharshan de Silva, Enoka M Corea. Distribution of Yersinia-like fimbrial (YLF) and Burkholderia thailandensis-like flagellum and chemotaxis (BTFC) gene clusters in Burkholderia pseudomallei isolates from Sri Lanka (REF NO.: WMC19.095). WMC 2019, 9th World melioidosis Congress, 15-18 October, Hanoi, Vietnam.
- Jayasinghearachchi HS, Corea EM, Muthugama TA, Francis VR1, Sathkumara HD, Krishnananthasivam S, De Silva AD. Molecular diversity among Burkholderia pseudomallei isolates from a case cluster of melioidosis reported following an extreme weather event in the Batticaloa District in 2015. National Health Research Symposium 2019. 22-23 October 2019. Sri Lanka.
- Muthugama TA, Jayasinghearachchi HS, Masakorala J, Silva D de, Corea EM. Genetic diversity among Burkholderia pseudomallei clinical isolates in Sri Lanka. Sri Lanka College of Microbiologist, August, 2020.
- Peththa Thanthirige Nipuni Danushika and H. S. Jayasinghearachchi, Co-inoculation of Pleurotus ostreatus with beneficial bacteria enhanced substrate utilization and mushroom production: An implication for the development of a novel bio-fertilizer. 21st International Forestry and Environment Symposium 2016 23rd & 24th September 2016 at Heritance Kandalama, Dambulla, Sri Lanka. doi:10.31357/FESYMPO.V21I0.3076
- Prevalence of duodenal ulcer promoting gene (dupA) of H. pylori in patients with duodenal ulcer disease and non-ulcer dyspepsia in North Indian population, Young Investigator Award session, 47th annual conference, Indian Society of Gastroenterology, 2006, November, Mumbai, India.
Nanotechnology in Medicine Society of Young Scientists, AIIMS, New Delhi 2007. - Intestinal bowel diseases, AIIMS, New Delhi, India, 2007. Indo-France workshop on Biohydrogen: from Basic Concepts to Technology, (Under the aegis. Indo French Center for the Promotion of Advanced Research, Jaypee, India, 2008
- Ocenotoga teriensis gen. nov. sp. nov., a novel thermophilc, bacterium belonging to the order Thermotogales, isolated from off-shore oil-producing well Bombayhigh in India, Association of Microbiologists of India, 2008
- Biohydrogen production from renewable source as feed stock, Annual conference of Association of Microbiologists of India, 2008. This year 49th AMI Conference is being held on November 18–20, 2008, at the University of Delhi, India,
- Microbial enhanced oil recovery, Annual Conference of Association of Microbiologists of India, 2008, This year 49th AMI Conference is being held on November 18–20, 2008, at the University of Delhi, India,
“Next-generation sequencing and bioinformatics for genomics & healthcare” at IIT, Madras, India. November, 2012. - Gunasekera D.T.W, Jayasinghearachchi, H.S., Gunawardana D. Bioremediation of cadmium by nitrogen-fixing actinobacteria residing in the root nodules of Casuarina equisetifolia. Sri Lanka Advancement of Science (SLAS), 2015
- Melioidosis: Unearthing a subterranean infection. 2nd South Asian Melioidosis Congress, Cinamom Lakeside Hotel, Colombo, Sri Lanka, 29-30 August 2017.
- Awareness workshop on ICGEB grant schemes and proposal writing for collaborative research programme (CRP) of ICGEB, 07th November,2017 Hotel Galadari, Colombo.
Graduate supervision
- Comparative study on the genetic diversity of the bimA allele and three selected gene clusters in clinical and environmental isolates of Burkholderia pseudomallei in the North Western Province of Sri Lanka and the influence of physicochemical parameters on the abundance of the bacterium in the environment. MPhil
- Detection of lpxO, bimA, fhaB3 genes, YLF/BTFC and LPSA gene clusters present in clinical isolates of Burkholderia pseudomallei from Sri Lanka using Real-Time PCR based molecular assay BSc. (Hons in Biomedical Sciences)
- Microbial diversity in nitrogen fixing nodules of Casuarina equisetifolia and its impact on plant growth and soil quality. BSc. (Special Degree in Microbiology)
- Co-inoculation of Pleurotus ostreatus with beneficial bacteria enhanced substrate utilization and mushroom production: An implication for the development of a novel bio-fertilizer. BSc. (Special Degree in Microbiology)
- Development of a novel fabric softener with antibacterial claim. BSc. (Special Degree)
- Getting accreditation for cosmetic testing. BSc. (Special Degree)
