Microbial Biotechnology Research at KDU CARE

 

The Microbial Biotechnology Laboratory, led by Prof. Himali S. Jayasinghearachchi, focuses on cutting-edge research in environmental and agricultural microbiology. Our state-of-the-art facility supports microbial solutions for bioremediation, biofertilizer development, and the production of valuable microbial metabolites. With a commitment to sustainability and innovation, our lab is driving microbial research for a cleaner environment and resilient agriculture.

For collaborations or inquiries, contact: Prof. Himali S. Jayasinghearachchi

jayasinghearachchihs@kdu.ac.lk

Bioremediation – Our research focuses on development of indigenous microbial consortia  to break down pollutants such as petroleum hydrocarbons, pesticides, and other xenobiotic compounds in the environment. These microbial processes contribute to environmental cleanup and sustainable waste management. We have developed a metabolically efficient microbial consortium for petroleum hydrocarbon and xenobiotic degradation in both aquatic and terrestrial environments. This approach leverages microbial biosurfactant-mediated degradation and utilizes an indigenous consortium isolated from marine environments.

 

 Biofertilizers – We develop microbial biofertilizers using beneficial fungi such as Trichoderma, mycorrhizal fungi, and plant endophytes. These enhance soil fertility, improve plant growth, and reduce reliance on chemical fertilizers, promoting sustainable agriculture.

Microbial & Enzyme Degradation of Synthetic Plastics –Biodegradation of Petroleum-Based Synthetic Plastics

 

Globally, 31.9 million metric tons of plastic waste accumulate annually, with 1.59 million metric tons in Sri Lanka alone—much of it reaching the ocean. The widespread release of microplastics contaminates marine ecosystems, posing risks to wildlife and human health through ingestion.

Microbial biotechnology: A sustainable solution for dealing with petroleum-based synthetic plastic pollution

We focus on identifying and characterizing indigenous microbial strains and depolymerases capable of breaking down petroleum-derived synthetic plastics. These microbes and enzymes are explored for bio-augmentation and bio-stimulation strategies to enhance the bioremediation of plastic-polluted environments.

 

 

Microbial Secondary Metabolites – We investigate the production of high-value microbial metabolites such as biosurfactants and pyocyanin for industrial, pharmaceutical, and environmental applications. Currently, our lab focuses on the production of biosurfactants using waste coconut oil as a sustainable raw material. These biosurfactants have potential applications in bioremediation, detergents, and pharmaceutical formulations.

Pyocyanin, a bioactive pigment produced by Pseudomonas spp., is studied for its antimicrobial properties. We explore its potential as an alternative to conventional antibiotics and its role in inhibiting pathogenic microorganisms.

 Industrial Enzymes & Biocatalysts – We study microbial enzymes such as depolymerases, lipases, and laccases for their role in industrial biocatalysis, biodegradation, and biotransformation. These enzymes have applications in waste management, biofuel production, textile processing, and pharmaceutical industries.

Precision Agriculture with AI Integration (Collaboration) – We explore the integration of microbial solutions with AI-driven precision agriculture to optimize crop health, improve yields, and enhance resource efficiency.

Our Facilities & Equipment

  • Biosafety Level 2 (BSL-2) Laboratory – Ensuring safe microbial research
  • Incubator Shaker – Optimizing microbial cultures
  • Digestion Block – Environmental sample processing
  • Centrifuges & Balance – Biomass separation & precise measurements
  • Combined pH & Conductivity Meter & Peristaltic Pump – Controlled experimental conditions
  • Vortex Mixer & Freeze Dryer – Sample preparation & long-term storage
  • Freezer – Microbial strain preservation
  • Light Microscope with Camera
  • Dissecting Microscope
  • Freeze Dryer Facility
  • Anaerobic Culture Facility
  • Gas Chromatography
  • Hot Air Oven
  • Cool Cabinet (4-8°C)
  • Tensiometer
  • Variable Volume Micropipettes
  • Filtration Units/Suction & Peristaltic Pumps – Lab-scale fermentation experiments
  • Greenhouse Facility

 

  • Access to:
  • High Performance Liquid Chromatography
  • Liquid Chromatography Mass Spectrometry
  • Autoclave Facility
  • Fume Hood
  • Laboratory Ultra Freezers
  • Real-Time PCR & Conventional PCR Facility
  • Gel Electrophoresis & Gel Documentation Facility